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KMID : 1161520010050040333
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Animal Cells and Systems
2001 Volume.5 No. 4 p.333 ~ p.339
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Genetic similarity and difference between common carp and Israeli carp (Cyprinus carpio) based on random amplified polymorphic DNAs analyses
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Yoon Jong-Man
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Abstract
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Common carp (Cyprinus carpio) and its aquaculture breed Israeli carp samples were obtained from two separate aquaculture facilities under the similar raising conditions during two years in the Kunsan National University, Korea. Genomic DNA was isolated from the common carp and Israeli carp for identification of genetic characteristics and genomic polymorphisms by polymerase chain reaction amplification of DNA using arbitrary primers. The arbitrary primer No. 21 (ACTTCGCCAC) yielded the highest number of fragments with the average of 15.0 among the primers used in Israeli carp. A total of 294 polymorphic products in common carp and 336 in Israeli carp were observed by random primers. The average number of polymorphic products generated by random RAPD primer No. 2 (GTAGAC?CCGT) showed 8.0 in Israeli carp. On average, each random RAPD primer produced 5.4 amplified polymorphic products in common carp and 6.2 in Israeli carp. An average genetic similarity (BS value) was 0.44±0.05 within the common carp and 0.32±0.04 within the Israeli carp. The degree of similarity frequency (BS) between two carps was 0.67 as generated by the primer No. 19 (GACGGATCAG). The average level of bandsharing was 0.57 ± 0.03 between the two carps. Accordingly, the two carp populations were genetically a little distant. The electrophoretic analysis of PCR?RAPD products showed middle levels of variation between the two carp populations. This result implies that the genetic diversity among intra?population may be higher when compared with that between the two carps. The RAPD polymorphism generated by these random primers might be used as a genetic marker for populations or lines identification in important aquacultural carp.
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KEYWORD
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Common carp, Israeli carp, Cyprinus carpio, Polymorphism, primer, PCR-RAPD
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